Journal: Toxicologic pathology
Article Title: Previously Diagnosed Reticulum Cell Hyperplasia in Decalcified Rat Bone Marrow Stain Positive for Ionized Calcium Binding Adapter Molecule 1 (Iba1) – a Monocytic/Macrophage Cell Marker
doi: 10.1177/0192623319890610
Figure Lengend Snippet: Details of the immunohistochemistry staining methods
Article Snippet: Therefore, only Iba1, and not ED2 and CD68, was used in the main study. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody CD68 ED2 Iba1 Lysozyme CD3 Pax5 Primary antibody source Abd Serotec AbD Serotec Wako Chemicals Dako Abcam Dako Catalog number MCA341GA MCA342R 019–19741 A0099 Ab5690 M7307 Lot number 0513 0214 SAN3725, LKN5648, LRH4161 0074097 GR232997 –1 20023427 Concentration 0.5 mg/ml 0.5 mg/ml 0.5 mg/ml 5 mg/ml 0.2 mg/ml 0.157 mg/ml Dilution 1:75 1:50 1:300 1:2000 1:100 1:50 Incubation time 1 hour 1 hour 1 hour 1 hour 1 hour 1 hour Negative control Mouse IgG1 Mouse IgG1 Rabbit IgG Rabbit Ig Fraction Rabbit IgG Mouse IgG1 Catalog number 557273 557273 N101 X0936 N101 557273 Source BD Biosciences BD Biosciences Calbiochem Dako Calbiochem BD Biosciences Positive controls used Rat spleen Rat spleen Rat brain Rat Intestine Rat spleen Rat spleen Antigen retrieval Pepsin, 3 min at 37C 0.01% trypsin, 20 min at 37C None Citrate buffer, pH 6.0 in Decloaker Rodent Decloaker (Biocare), pH 6.0 in Decloaker Citrate buffer, pH 6.0 in Decloaker Secondary antibody Horse anti-mouse ImmPress anti-mouse Ig HRP, Goat anti-rabbit Donkey anti-rabbit Rabbit on Rodent Horse anti-mouse Source and catalog number Vector, BA-2001 Vector, MP-7422–15 Vector, BA-1000 Jackson Immunoresearch, 711–065–152 HRP, Biocare RMR622 Vector, BA-2001 Lot number Z0421 ZA1013 ZB1007, Z1203, ZA0924 124459 121415 Z0421 Concentration 1.5 mg/ml N/A 1.5 mg/ml 1.2 mg/ml N/A 0.5 mg/ml Dilution 1:1000 Ready to use 1:300 1:500 Ready to use 1:500 Incubation time 30 minutes 30 minutes 30 minutes 30 minutes 30 minutes 30 minutes Additional reagents ABC label N/A ABC label ABC label N/A ABC label Source and catalog number Vector, PK-7100 Vector, PK-7100 Vector, PK-7100 Vector, PK-7100 Dilution Ready to use Ready to use Ready to use Ready to use Incubation time 30 minutes 30 min at RT 30 minutes 3 minutes Chromogen DAB DAB DAB DAB DAB DAB Open in a separate window Details of the immunohistochemistry staining methods
Techniques: Immunohistochemistry, Staining, Concentration Assay, Incubation, Negative Control, Plasmid Preparation
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